DDDDK-Tag(binds to flag sequnence) rabbit pAb
簡(jiǎn)要描述：DDDDK-Tag(binds to flag sequnence) rabbit pAb宿主: Polyclonal, Rabbit,IgG反應性: Species independent應用: WB;ELISA;IP;IF
產(chǎn)品型號： YP-Ab-17192
所屬分類(lèi)：標簽內參抗體
更新時(shí)間：2023-10-19
詳細說(shuō)明：
DDDDK-Tag(binds to flag sequnence) rabbit pAb
抗體，也叫免疫球蛋白（Ig），是一種能特異性結合抗原的糖蛋白，而抗原是在易感染動(dòng)物體內引發(fā)抗體產(chǎn)生的物質(zhì)。在體內，抗體是由于外源性分子的侵襲而產(chǎn)生的?？贵w以一個(gè)或者多個(gè)Y字形單體存在，每個(gè)Y字形單體由4條多肽鏈組成，包含兩條相同的重鏈和兩條相同的輕鏈。輕鏈和重鏈是根據它們的分子量大小來(lái)命名的。Y字形結構的頂端是可變區，為抗原結合部位。任何一個(gè)抗體的輕鏈都可以分為κ或λ型（基于小分子多肽結構上的差異），每一個(gè)抗體的重鏈則決定了它的類(lèi)或型。
產(chǎn)品詳情
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
免疫原詳細信息
DDDDK synthetic peptide conjugated to KLH.
相關(guān)試劑
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
背景
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
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備注：圖片僅供參考。僅供科研，不可用于臨床。
